生何首乌多糖的单糖组成及清除羟自由基的活性测定

摘要

Characterize the monosaccharide composition,molecular weight and scavenging hydroxyl free radical activity of polysaccharides from Polygonum mulitiflorum Radix.Ultrasonic extraction of polysaccharide from Polygonum mulitiflorum Radix is isolated by DEAE-650C.The molecular weights are characterized by high performance gel permeation chromatography.The monosaccharide compositions are analyzed by HPLC applied to PMP precolumn derivation.Through the Fenton reaction produces (·OH)to evaluate the ability of polysaccharide from Polygonum mulitiflorum Radix on scavenging hydroxyl free radical(·OH).Polysaccharide from Polygonum mulitiflorum Radix is constituted by twocomponents named PMPS1 and PMPS2.The relative molecular weight of PMPS1 is 87 449 and PMPS2 is 132 499.PMPS1 mainly is composed by galactose,glucose,arabinose and their molar ratio is 1:89.1:3.2.PMPS2 mainly is composed by the galactose,galacturonic acid,glucose,arabinose and their molar ratio is 2.5:1:395.8:3.8;The EC50 of PMPS1and PMPS2 which clearance on scavenging hydroxyl free radical are 2.86 mg · mL-1,1.13 mg · mL-1.Polysaccharide from Polygonum mulitiflorum Radix is constituted by two-components,theyent.The ability of acidic polysaccharide PMPS2 on scavenging hydroxyl free radical hydroxide is stronger than PMPS1%测定生何首鸟多糖的相对分子质量与单糖组成以及生何首乌多糖对羟自由基清除能力.用超声波辅助法提取生何首乌多糖；采用DEAE-650C纤维素拄分离、纯化生何首乌多糖；高效凝胶过滤色谱法测定分离组分的相对分子质量；利用PMP柱前衍生化HPLC分析单糖组成；通过Fenton反应产生(·OH)评价生何首鸟多糖对羟自由基的清除能力.从生何首乌多糖中分离纯化出2个组分,分别命名为PMPS1,PMPS2,相对分子质量分别为87 449和132 499,PMPS1主要由甘露糖、葡萄糖、阿拉伯糖组成,摩尔比为1:89.1:3.2；PMPS2主要由甘露糖、半乳耱醛酸、葡萄糖、阿拉伯糖组成,摩尔比为2.5:1:395.8:3.8,PMPS1和PMPS2清除羟自由基的EC50值分别为2.86 mg·mL-1,1.13 mg·mL-1.生何首乌多糖的两个组分主要是由葡萄糖组成的杂多糖,其相对分子质量和单糖组成均不同,酸性多糖组分PMPS2对羟自由基的清除能力较强.