The β-receptor blockers are a class of the chiral drugs for the treatment of cardiovascular diseases and most of them present two enantiomers (R & S),which often exhibit different pharmacology,toxicology and pharmacokinetics.In this paper,a new 2,4-dinitrobenzene ether-β-cyclodextrin-bonded SBA-15 chiral stationary phase (NESP) was reported.The performance of NESP were systematically studied with four kinds of β-blockers as chiral solute probes by high performance liquid chromatography (HPLC).The effects of mobile phase,temperature and flow rate on the enantioseparation of esmolol were also investigated.It was confirmed that the separation of esmolol is enthalpy-controlled by the related thermodynamic parameters analysis.The results show that the NESP has excellent chiral separation ability for the β-blockers,such as esmolol,metoprolol,atenolol and propranolol.%β-受体阻滞剂是一类治疗心脑血管类疾病的手性药物,大多有2个对映异构体,其药理学、毒理学及药代动力学具有明显的差异.本文合成了2,4-二硝基苯醚-β-环糊精键合SBA-15手性固定相(NESP),以4种β-受体阻滞剂为溶质探针,在高效液相色谱条件下较系统地研究了该固定相对这类药物的手性分离能力.以艾司洛尔为例探讨了流动相组成、温度变化、流速变化对手性分离的影响,通过研究热力学参数变化,证实艾司洛尔的分离是焓驱动过程.研究表明,所制备的固定相对这类药物如艾司洛尔、美托洛尔、阿替洛尔和普萘洛尔具有良好的快速手性拆分能力.
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