目的：探讨脑慢性低灌注白质损伤后大鼠脑周细胞RGS5（Regulators of G protein signaling 5， RGS5）、PDGFR-β（ Platelet-derived growth factor receptor-beta， PDGFR-β）的表达。方法：采用蛋白免疫印迹法检测脑慢性低灌注白质损伤大鼠脑周细胞RGS5、PDGFR-β在不同时间段（术后第7、14、30、60d）表达变化。结果：（1） RGS5蛋白在对照组中有稳定的表达，与对照组相比，模型组在术后第14d明显下调（P＜0．05），第30d表达增强明显（P＜0．05），第60d达到高峰（P＜0．05）；（2）PDGFR-β蛋白在对照组中也有稳定的表达，与对照组相比，模型组在术后第30d表达至高峰（P＜0．01），第60d有所下降，但仍高于正常（ P＜0．05）。结论：正常生理条件下，脑周细胞中有一定量的 RGS5和PDGFR-β表达；脑慢性低灌注白质损伤后期阶段，脑周细胞中RGS5和PDGFR-β蛋白表达增强，且PDGFR-β先于RGS5达到表达高峰，两者表达存在时相关系。%Objective:To investigate the expression of RGS5、PDGFR-βin the brain white matter pericyte afte chronic cerebral hy-poperfusion injury.Methods:The Western Blot method was used to detect the alterations of the RGS5、PDGFR-βproteins at different periods of time (in the postoperative 7th,14th,30th,60th day ) in the brain white matter pericytes of chronic cerebral hypoperfusion suffering rats.Results:(1)Expression of RGS5 protein is constant in the sham operation group.Expression of the RGS5 protein in the model group has decreased in the postoperative 14th day, compared with the control group (P<0.05), and the expression is obviously enhanced in the 30th day , the 60th day to peak (P<0.05); (2) The PDGFR-βprotein is also constant expression in the sham group,compared with the control group, the model group's PDGFR-βprotein's relative expression reached the peak in the 30th day ( P<0.01), and lowered in the 60th day, which was still above the normal range (P<0.05).Conclusion:Under normal physiological conditions, the brain pericytes have a certain amount of RGS5 PDGFR-βexpression; the expression of RGS5、PDGFR-βare en-hanced in the brain white matter pericyte afte chronic cerebral hypoperfusion injury late stage, and PDGFR-βexpression reached a peak before the RGS5's, so there is a phase relationship between the expression systems.
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