依普黄酮对破骨细胞活性及成骨细胞增殖分化作用的研究

摘要

To explore the dosage effect of ipriflavone on the differentiation and bone resorption activity of osteo-clast, osteoclast module was obtained from 1α, 25-(OH)2VitD3 -induced rabbit bone marrow cells. Also, MC3T3-E1subclone 14 cell model was used to explore the dosage effect of ipriflavone on the proliferation and differentiation of osteoblasts. Results showed that 10-5 mol/L,10-6 mol/L,10-7 mol/L,10-8 mol/L,10-9 mol/L,and 10-10 mol/L ipriflavone significantly inhibited differentiation and bone resorption activity of osteoclast (10-8 mol/L for the most), and 10-6 mol/L,10-7 mol/L,10-8 mol/L,10-9 mol/L,and 10-10 mol/L highly pro-moted proliferation and differentiation of MC3T3 - E1Subclone 14 cells (10-10 mol/L for the most).%采用1α,25-( OH)2 VitD3诱导兔骨髓细胞形成破骨细胞模型研究不同浓度依普黄酮对破骨细胞分化及骨吸收活性的影响；采用MC3T3-E1Subclone14(小鼠颅顶前骨细胞亚克隆14)细胞模型研究不同浓度依普黄酮对成骨细胞增殖分化作用的影响。结果表明10-5,10-6,10-7,10-8,10-9,10-10 mol/L的依普黄酮均能显著性抑制破骨细胞分化及骨吸收活性(P<0．05)其中10-8 mol/L的依普黄酮抑制作用最好。10-6,10-7,10-8,10-9,10-10 mol/L的依普黄酮均能显著性促进MC3T3-E1Subclone 14细胞的增殖和分化(P<0．05),10-10 mol/L的依普黄酮促进作用最好。