miR-145在浆液性卵巢癌中的表达及对卵巢癌SKOV3细胞增殖的影响

摘要

Objective:To detect the expression of miR-145 in serous ovarian carcinoma and SKOV3,and to ex-plore its effect on the proliferation of ovarian cancer cell SKOV3 and its possible molecular mechanism.Methods:The expression of miR-145 in 43 serous ovarian carcinoma tissues and adjacent tissues was detected by real-time quan-titative PCR.The real-time quantitative PCR was used to detect the expression of miR-145 in normal ovarian serous gland cells and SKOV3 cells.The miR-145 agomir was synthesized and then the miR-145 agomir and controls were transfected into SKOV3 cells respectively.The MTT assay was uesd to detect the proliferation of SKOV3 cells,explo-ring its effect on the proliferation of ovarian carcinoma cell SKOV3.Results:The results of real-time quantitative PCR showed that the expression of miR-145 in serous ovarian carcinoma tissues was lower than that in adjacent tis-sues.Compared with normal cells the expression of miR-145 was decreased in SKOV3 cells.MTT results show that the proliferation of SKOV3 cells transfected with miR-145 angomir were significantly inhibited.Conclusion:miR-145 is decreased in serous ovarian carcinoma tissues and SKOV3,miR-145 can inhibit the proliferation of ovarian cancer cells,miR-145 is closely related to the prognosis of patients with serous ovarian carcinoma,its increased ex-pression suggests a good prognosis.miR-145 is expected to become a new biology marker for early diagnosis and prognosis.%目的:检测miR-145在浆液性卵巢癌组织及卵巢癌SKOV3细胞中的表达情况,探讨其对卵巢癌SKOV3细胞增殖的影响及其可能的分子机制.方法:利用实时定量PCR技术检测43组浆液性卵巢癌患者癌及癌旁组织中miR-145的表达情况;利用实时定量PCR技术检测正常卵巢浆液腺细胞及SKOV3细胞中miR-145的表达情况;合成miR-145的模拟物miR-145 agomir,将该模拟物及对照分别转染入SKOV3细胞中,应用MTT法检测细胞增殖情况,明确miR-145对卵巢癌SKOV3细胞增殖的影响.结果:实时定量PCR结果显示,与癌旁组织相比,miR-145在浆液性卵巢癌患者癌组织中表达含量降低;与正常细胞相比,miR-145在SKOV3细胞中表达含量降低;MTT结果表明转染了miR-145 angomir的SKOV3细胞增殖受到明显的 抑制.结论:miR-145在浆液性卵巢癌组织及SKOV3细胞中均表达含量降低,miR-145可以抑制卵巢癌细胞增殖,miR-145与卵巢癌患者预后密切相关,其含量增高提示好的预后,有望成为新的生物学标志物用于卵巢癌早期诊断及判断预后.