Objective :To evaluate the influence of IL - 1 β on the expression of MMP -2 of HFL cells in three dimensional culture collagen gels. Methods: HFL cells were cultured in three - dimensional culture collagen gels. HFL cells were randomly divided into control and experimental groups. Cells of the control group were cultured in DMEM media without IL - 1β. Cells of experimental groups were cultured in DMEM media with different concentrations of IL - 1β( 10,20 ng/ml ) , respectively. The phase contrast microscope was used to observe the morphology of cells. After 24 ,72 hours, harvested the supernatant, MMP - 2 were detected by gelatin zymography. The proliferation of HFL cell stimulated by IL - 1β was examined by tetrazolium salt ( MTT )assay. Results:ln three - dimensional co - culture , HFL cells were growing in gel - culture, and it didn't proliferate, and all of the cells were growing in singles. The expression of MMP - 2 was different in different IL - 1β concentration, and the stimulus was enhanced with the increasing of IL - 1β doses. IL - 1β can enhance HFL proliferation in a concentration - dependent Manner. The different concentrations of IL - 1β had different effects on proliferation of HFL ( P ＜ 0. 05 ), the proliferation ability was dose - time dependent. Conclusion : IL - 1β may enhance the invasion and metastasis of lung cancer through up - regulating the secretion and of MMP - 2, and enhance HFL proliferation.%目的:探讨三氧化二砷(As2O3)对人肝癌SMMC-7721细胞黏附、侵袭、转移能力的影响以及其机制.方法:采用MTT法观察人肝癌SMMC-7721细胞经As2O3作用前后对基底膜黏附能力的影响;Transwell膜侵袭系统观察SMMC-7721细胞经As2O3作用前后游走与穿透基底膜能力的改变.免疫细胞化学方法检测人肝癌细胞经 As2O3作用前后CD44V6表达的改变.结果:As2O3能显著抑制人肝癌SMMC-7721细胞与marigel的黏附、抑制细胞游走与穿透基底膜的作用(P＜0.05),能抑制人肝癌细胞CD44v6的表达(P＜0.05).结论:As2O3具有抑制人肝癌细胞的侵袭、转移能力,其抑制作用可能与CD44v6的表达下调有关.
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