异丙酚对人肝癌细胞侵袭转移能力及上皮间质转化的影响

摘要

Objective To investigate the influence of propofol on invasion , metastasis and epithelial -mesenchy-mal transition (EMT) of human hepatocellular carcinoma cells .Methods Hepatocellular carcinoma cells ( HCCLM3, QGY-7703, SMMC-7721) were stimulated with propofol in gradually increasing dosages (5 μg/mL, 10 μg/mL and 20 μg/mL).The effects of propofol on the migration and invasion of hepatocellular carcinoma cells were assessed by wound healing assay, trans-well matrix invasion assay and three -dimensional spheroid invasion assay .HCCLM3 cells were stimulated with propofol of 20 μg/mL.Western blot and immunofluorescence assay were used to detect the expression of EMT makers (E-cadherin and Vimentin ).Results With the increase of propofol concentration , wound healing assay showed that the migration ability of hepatocellular carcinoma cells significantly decreased ( P＜0.05); trans-well inva-sion experiments and three -dimensional culture experiments showed that the invasive ability of hepatocellular carcinoma cells significantly decreased (P＜0.05).The expression of E-cadherin was up-regulated and the expression of vimen-tin was down-regulated in HCCLM3 cells after propofol stimulation of 20 μg/mL (P＜0.05).Conclusion In vitro, propofol can suppress the migration and invasion of HCC cells with concentration -dependent effect , which may be corre-lated to the inhibition of EMT.%目的 探讨异丙酚对人肝癌细胞侵袭转移能力及上皮间质转化(EMT)发生的作用.方法 用逐渐增量(5、10、20 μg/mL)的异丙酚刺激肝癌细胞( HCCLM3、QGY-7703、SMMC-7721)后,通过体外的细胞功能学实验(划痕实验、Transwell、三维培养实验)检测异丙酚对肝癌细胞迁移和侵袭能力的影响. 20 μg/mL的异丙酚刺激肝癌细胞,Western blot 与免疫荧光实验检测刺激后 HCCLM3 细胞 EMT 相关标志物(E-cadherin、Vimentin)的表达情况.结果 随着异丙酚浓度的提高,划痕实验显示肝癌细胞的迁移能力下降,Tr-answell侵袭实验和三维培养实验显示肝癌细胞侵袭能力下降(P＜0.05). 20 μg/mL异丙酚刺激HCCLM3细胞后,肝癌细胞的EMT得到抑制,上皮标志物E-cadherin表达上调,而间质标志物Vimentin 表达下调( P＜0.05).结论 在体外细胞模型中,异丙酚5～20 μg/mL呈剂量依赖性抑制肝癌细胞的转移与侵袭,机制可能与其抑制EMT的发生有关.