Tim-3在恶性黑色素瘤患者外周血CD8+T细胞上的表达及临床意义

摘要

Objective:To explore the role of T cell immunoglobulin and mucin domain molecules-3 (Tim-3) in malignant melanoma progression via detecting Tim-3 expression on CD8 + T Cells from peripheral blood of malignant melanoma patients.Methods:Detect Tim-3 expression on CD8 + T cells from peripheral blood of 42 malignant melanoma patients and 38 healthy volunteers using flow cytometry,and analyze its relationship with clinical pathological features of the malignant melanoma patients.Results:The expression of Tim-3 CD8 +T cell existed in both malignant melanoma patients and healthy volunteers,however,the proportion of Tim-3 CD8 + T cell in malignant melanoma group(15.89-± 6.49) ％ was significantly higher than the control group (3.27 ± 2.16) ％,P ＜ 0.01.Higher expression of Tim-3 CD8 + T cells in peripheral blood mononuclear cell was related to ulceration,lymph node metastasis,TNM stage,and distant metastasis in malignant melanoma patients (P ＜ 0.01),but not related to gender and age(P ＞0.05).Conclusion:Malignant melanoma patient's peripheral blood CD8 +T cell expressed higher levels of Tim-3 compared to healthy donors,moreover,the expression level had positive correlation with TNM stage.Tim-3 may be a valuable molecular marker and an important therapeutic target.%目的:本研究应用流式细胞术检测恶性黑色素瘤患者外周血CD8+T细胞中T细胞免疫球蛋白及黏蛋白-3(Tim-3)的表达情况,探讨Tim-3在恶性黑色素瘤进展中的作用.方法:应用流式细胞仪检测42例恶性黑色素瘤患者及38例健康体检者外周血CD8+T细胞上Tim-3的表达,同时分析其与恶性黑色素瘤患者临床病理特征的关系.结果:Tim-3在恶性黑色素瘤患者及健康体检者外周血CD8+T细胞中均有不同程度的表达,恶性黑色素瘤组Tim-3表达量为(15.89±6.49)％,健康对照组的表达量为(3.27±2.16)％,差异具有统计学意义(P＜0.01).Tim-3在CD8+T细胞中的表达水平与恶性黑色素瘤患者有无溃疡、淋巴结转移、临床分期、远处转移有关(P＜0.01),而与性别、年龄、Breslow分度无关(P＞0.05).结论:Tim-3在恶性黑色素瘤患者外周血CD8+T细胞上高水平表达,其表达水平与临床分期呈正相关,并有望成为恶性黑色素瘤早期预测及治疗的重要靶标.