B细胞非霍奇金淋巴瘤TCR基因重排及CDR3谱型分析

摘要

Objective To analyze the clonal gene rearrangement and complementarity determining region 3(CDR3)Repertoire of TCR β-chain in fine needle aspiration biopsy(FNAB)specimens of B-cell lymphoma.Methods The TCR CDR3 region genes of 24 TCR Vbeta subfamilies were amplified by utilizing RT-PCR technology,and the CDR3 lengths of TCR β-chain were analyzed with GeneScan technology for 4 healthy individuals and 3 patients with B-cell lymphoma.The clonality of T cells presumed by spectratyping was further confirmed by CDR3 sequencing.Results TCR β-chain presented specific repertoire skewing in 3 cases,and only 2-5 TCR Vbeta subfamily T cells were identified,respectively.Clonal expanded T cells,including oligoclonal.oligoclonal trend patterns,in one or more Vbeta subfamilies were found in all cases,The oligoclonal expanded T cells have different CDR3 amino acid sequences.Conclusion There were characteristic T cells clone proliferation and selected usage of TCR Vbeta subfamily T cells could be found in 3 cases with B-cell lymphoma.The sequences of CDR3 in different TCR clone proliferation ale mostly different.%目的 分析B细胞淋巴瘤穿刺标本中T细胞受体(TCR)B链克隆性基因重排及互补决定区3(CDR3)谱型.方法 应用RT-PCR扩增TCR VB24个亚家族的CDR3区基因,并经基因扫描确定T细胞克隆性,对提示单克隆或寡克隆增生亚家族的PCR产物进行测序.结果3例淋巴瘤患者TCR存在限制性取用,仅表达2～5个V β亚家族.所有患者均存在克隆性增生T细胞,增生形式包括寡克隆及寡克隆增生趋势.CDR3区序列分析证实增生的T细胞克隆具有不同的氨基酸序列.结论 B细胞淋巴瘤患者存在T细胞克隆性增生,其TCRVβ呈限制性取用,不同克隆T细胞的CDR3序列不同.