肠道菌群利用去淀粉麦麸体外发酵产短链脂肪酸

摘要

Non-digestible carbohydrate could be utilized to different extent by different species of human gut microbiota. In the present study, we used destarched wheat bran (DWB) as carbon soruce for in vitro fermentation production of short chain fatty acids (SCFAs) by gut microbiota from human feces. The types and concentrations of SCFAs in the fermentation broth were also determined. The results showed that all of the gut microbiota, including non-probiotics, could utilize DWB to produce different types and concentrations of SCFAs. Except Lactobacillus, the other gut microbiota mainly produced acetic acid.Lactobacillus produced more propionic acids, but only a little amount of acetic acid. According to the yields at the end of fermentation, DWB attributed to the production of SCFAs in the order of acetic acid ＞propionic acid ＞ lactic acid and isobutyric acid ＞ butyric acid.%肠道菌群中不同菌对同一种不消化性碳水化合物的利用程度不同.本实验利用去淀粉麦麸(destarched wheat bran,DWB)作为碳源,用人体粪便作为肠道细菌来源,对不同肠道菌群进行体外发酵培养,在不同时间取其发酵液,测定发酵液内SCFA的种类和数量的变化.结果显示,肠道中各种细菌(包括非益生菌)均能利用DWB产生短链脂肪酸(short chainfatty acid,SCFA),但其种类和数量各有差异;除乳酸菌外,各类菌产生的SCFA都主要是乙酸,其次是丙酸.乳酸菌产乳酸的能力强于其他菌,产乙酸的能力则弱一些.从发酵末期的产量来比较,DWB对乙酸的贡献值最大,丙酸其次,乳酸和异丁酸差不多,丁酸的贡献值最小.