不同浓度酒精灭活后的国人成骨肉瘤细胞超微结构的变化

摘要

Objective：To study the ultrastructure changes of osteosarcoma cells(OS-732) inactivated by alcohol of two different concentrations and clinical application value.Methods：Osteosarcoma cells(OS-732) were inactivated by 75% and 95% alcohol and observed by light and electron microscope.And its viability was detected by MTT method. Results：Cell internal structure changed significantly and irreversible damage formed. MTT method proved that inactivated cells had no viability.Conclusion：These two inactivation methods were effective. Cell internal enviroment was damaged very seriously and cell was led to death. These two inactivation methods could provide choices for clinical limb protective operation.%目的：探讨两种不同浓度的酒精灭活骨肉瘤细胞(OS-732)后细胞内部结构变化和临床应用价值。方法：75%和95%酒精灭活骨肉瘤细胞，光镜和电镜下观察细胞形态，MTT法检测细胞活性。结果：灭活后的细胞内部结构发生明显改变，形成不可逆性损伤。MTT法检测证明处理后的肿瘤细胞已无生存能力。结论：两种浓度的酒精对骨肉瘤细胞的灭活是有效的，为临床上骨肉瘤的保肢治疗提供良好的选择。