目的：建立HPLC法测定小儿肺热咳喘颗粒中2种活性成分的含量。方法色谱柱为PLATSILTM ODS色谱柱（250 mm ×4．6 mm，5μm），柱温30℃，流动相为甲醇-0．1％磷酸水溶液，采用梯度洗脱：0～5 min，35％～50％A；5～10 min，50％～75％A；10～17 min，75％～90％A；17～25 min，90％A；25～30 min，90％～35％A；30～40 min，35％A，检测波长为327 nm，流速为1 mL／min，柱温30℃。结果绿原酸、黄芩苷的线性范围分别为0．084～1．260μg （R＝0．9995）和0．242～3．630μg（R＝0．9996），此范围内与峰面积呈良好的线性关系。结论该法检测效率高，专属性强，重复性好，可用于小儿肺热咳喘颗粒中绿原酸和黄芩苷的定量测定。%Objective To establish a method for simultaneous determination of chlorogenic acid and baicalin in Xiao'er Feire Kechuan Granules .Methods Using PLATSILTM ODS (250 mm ×4.6 mm,5μm) column with metha-nol (A) -0.1%phosphoric acid (B) gradient:0 ~5 min,35%~50%A;5~10 min,50%~75%A;10~17 min, 75%~90%A;17~25 min,90%A;25~30 min,90%~35%A;30~40 min,35%A,a flow rate of 1 mL/min,detec-tion wavelength:327 nm, the column temperature was 30 ℃.Results The results showed that the linear range of chlorogenic acid and baicalin was 0.084~1.260μg (R=0.999 5) and 0.242~3.630μg (R=0.9996) respec-tively ,and the sample amout and the peak area had a good linear relationship .Conclusion The method is accurate , simple,with good reproducibility and stability ,and is suitable for quality control of Xiao'er Feire Kechuan Granules .
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