Improved RP-HPLC (Reversed-Phase, High-Performance Liquid Chromatography) Method for Determining Nitroaromatics and Nitramines in Water

摘要

A protocol was developed for determining nitroaromatic and nitramine explosive by reversed-phase, high-performance liquid chromatography on an LC-18 column. The method employs dilution of an aqueous sample 1:1 with methanol, filtration through a 0.5 micro meter Millex-Sr filter, separation on the LC-18 column using a 1:1 water-methanol eluent, and determination by UV-254 nm. A careful comparison was made with an earlier standard protocol, which uses separation on an LC-8 column with a 50:38:12 water-methanol-acetonitrile eluent. Overall, the new procedure provides better separation for a wider range of analytes and equivalent recovery for all analytes tested. The new procedure is particularly effective at separating TNT from tetryl, and it allows analysis of water and soil extracts using a single column and eluent combination. Analytical methods, Contaminated soils, Explosives, High-performance liquid, Chromatography, Soils. (JES)