HPLC-MS/MS与ICP-MS分别测定血液中14种全氟化合物及常见金属元素

摘要

为了解广东儿童血液中全氟化合物(PFCs)和常见金属元素的含量水平,为儿童健康评估提供数据,该文采用蛋白沉淀法提取样品,高效液相色谱-串联质谱(HPLC-MS/MS)检测样品中14种PFCs.同时,以石墨全自动消解仪消解血液样品,电感耦合等离子体质谱(ICP-MS)检测血液中钙(Ca)、镁(Mg)、铁(Fe)、锌(Zn)、铜(Cu)和锰(Mn)元素.结果显示,儿童血液中14种PFCs被普遍检出,其中全氟辛基磺酸(PFOS)和全氟辛基羧酸(PFOA)的检出率及浓度最高,但在一些个体中发现较高浓度的其它碳链PFCs.PFCs浓度有随着年龄增长而F降的趋势,但除了全氟丁基磺酸外,无性别差异.另一方面,儿童血液中检出的Ca、Mg、Fe、Zn、Cu和Mn含量在正常范围内,未发现有性别差异.研究显示PFCs普遍存在儿童血液中,儿童PFCs暴露水平的研究应引起重视.在今后的监测中,不仅要关注PFOS和PFOA,也要关注其它碳链PFCs.%The aim of this paper was to understand the concentrations of perfluorinated compounds (PFCs) and common metal elements in children's blood and provide data for the assessment of children's health.The samples were extracted by protein precipitation,and then detected by high liquid chromatography-electrospray-mass spectrometry (HPLC-MS/MS).Meanwhile,the samples were digested with graphite automatic instrument,and then calcium(Ca),magnesium(Mg),iron(Fe),zinc(Zn),copper(Cu) and manganese(Mn) in samples were determined by inductively coupled plasma-mass spectrometry(ICP-MS).The PFCs in children's blood were widely detected.The frequencies and concentrations of perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) were the highest,whereas some other PFCs with higher concentrations were found in some blood samples.A negative correlation was found between the concentrations of PFCs and the children's age.There was no gender difference in the concentrations of PFCs except perfluorobutane sulfonate.The concentrations of Ca,Mg,Fe,Zn,Cu and Mn were in normal range.The PFCs in children's blood were widely determined.Concerns regarding children exposure to PFCs are needed.Focus should be paid not only on PFOS and PFOA,but also on other PFCs in the future monitoring.