卵巢癌超声造影参数与血管新生、癌细胞生长的相关性研究

摘要

目的:研究卵巢癌超声造影参数与血管新生、癌细胞生长的相关性.方法:选择2015年2月～2017年6月期间在四川省凉山州第二人民医院接受手术切除的卵巢肿瘤患者作为研究对象,根据术后病理结果分为肿瘤性质为卵巢癌的卵巢癌组以及肿瘤性质为良性肿瘤的对照组.术前进行超声造影检查并测定达峰时间TTP、增强强度EI;术后收集肿瘤病灶并测定血管新生分子、细胞增殖分子、细胞侵袭分子的蛋白含量.结果:卵巢癌组卵巢癌病灶组织的EI显著高于对照组良性肿瘤病灶组织(P＜0.05),TTP显著低于对照组良性肿瘤病灶组织(P＜0.05);卵巢癌病灶组织中VEGFR1、Tie-2、CGB5、NFATc1、CyclinD1、FUNDC1、IGFBP2、IGFBP3、ZEB1的蛋白含量显著高于良性肿瘤病灶组织且与EI水平呈正相关(P＜0.05),与TTP水平呈负相关(P＜0.05);TNFSF15、Fibulin-5、ELF5、p27Kip1、p21、FN14、E-cadherin的蛋白含量显著低于良性肿瘤病灶组织(P＜0.05),EI水平呈负相关(P＜0.05),与TTP水平呈正相关(P＜0.05).结论:卵巢癌超声造影参数能够反应病灶内血管新生的程度及癌细胞浸润性生长的活力.%Objective:To study the correlation of contrast-enhanced ultrasonography parameters of ovarian cancer with angiogenesis and cancer cell growth.Methods:Patients with ovarian tumor who underwent surgical resection in the Second People's Hospital of Liangshan Yi Autonomous Prefecture between February 2015 and June 2017 were selected as the research subjects and divided into the ovarian cancer group with ovarian cancer and the control group with benign tumor according to the postoperative pathological results of tumor.Contrast-enhanced ultrasonography was performed before operation to determine the time to peak TTP and enhanced intensity EI;the tumor lesion was collected after operation to determine the protein levels of angiogenesis molecules,cell proliferation molecules and cell invasion molecules.Results;The EI of ovarian cancer lesion tissue of ovarian cancer group was significantly higher than that of benign tumor lesion tissue of control group whereas the TTP was significantly lower than that in benign tumor lesion tissue of control group;VEGFR1,Tie-2,CGB5,NFATc1,CyclinD1,FUNDC1,IGFBP2,IGFBP3 and ZEB1 protein levels in ovarian cancer lesion tissue were significantly higher than those in benign tumor lesion tissue,positively correlated with EI level and negatively correlated with TTP level;TNFSF15,Fibulin-5,ELF5,p27Kip1,p21,FN14 and E-cadherin protein levels were significantly lower than those of benign tumor lesion tissue,negatively correlated with EI level and positively correlated with TTP level.Conclusion:The contrast-enhanced ultrasonography parameters of ovarian cancer can reflect the degree of angiogenesis and the viability of the invasive growth of cancer cells within lesions.