Amorphous calcium carbonate binding protein (ACCBP), which is abstracted from a marine mullusc Pinctada fucata, performs notably in biomineralization, in vitro. But its low existence limited the related research. In this research, rACCBP was used, which was expressed in yeast, instead of ACCBP in order to study its functions on MC3T3-E1 cell. First, the reasonable concentration and duration for incubation of rACCBP were studied. rACCBP down-mediated MC3T3-E1 differentiation. Medium with DMEM containing 0.001 μg/mL rACCBP, cultured for 8 days, ALP activity was only 7.187 nmol /min/mg, much lower than 11.917 nm pNPP/min/mg protein. Moreover, interactions between rACCBP, dexamethane and ascorbic acid were studied on three levels, none, low and high. rACCBP also seems to restrain their stimulations on MC3T3-E1 differentiation.%以MC3T3-E1细胞系为实验材料,探讨了真核表达的重组蛋白rACCBP对成骨细胞的作用.结果表明:rACCBP对MC3T3-E1的分化具有抑制作用,用含有0.001 g/mL rACCBP的DMEM培养液,培养8d后细胞的ALP活性仅为7.187 U/mg,远低于参照的11.917 U/mg;rACCBP削弱了dexamethane和ascorbic acid对MC3T3-E1的诱导作用.
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