奥卡西平对癫痫幼鼠海马神经元神经细胞黏附分子表达的影响

摘要

目的观察奥卡西平（OXC）对慢性癫痫幼鼠海马神经元中神经细胞黏附分子（NCAM ）表达的影响。方法将50只21日龄SD大鼠随机分为5组：生理盐水（NS）组、戊四氮（PTZ）组、OXC低剂量（100 mg/kg ， LDOXC）组、OXC中剂量（200 mg/kg ，MDOXC）组、OXC高剂量（300 mg/kg ，HDOXC）。建立不同剂量的OXC干预癫痫幼鼠模型。连续用药21 d ，观察幼鼠体质量、行为学表现；免疫组织化学法观察 NCAM 阳性细胞的表达；Real-time PCR检测海马组织中NCAM mRNA的表达。结果 LDOXC、MDOXC、HDOXC组癫痫发作时间延长，发作程度减低（P ＜0．01）；LDOXC、MDOXC、HDOXC组海马组织中NCAM 阳性细胞、NCAM mRNA的表达降低（P＜0．05）。结论 OXC可抑制癫痫幼鼠海马神经元中NCAM 的表达，并存在一定的剂量依赖性。OXC可能通过减少NCAM的表达，抑制PTZ致幼痫鼠海马神经元发生，减轻癫痫引起的脑损伤。%Objective To explore the effects of oxcarbazapine(OXC)on the expression of NCAM in hippocampus of immature rats with epilepsy . Methods A total of 50 male SD rats around 19 to 21 days of age were randomly allocated into 5 groups :normal saline (NS)group ,pentylenetetrazole (PTZ) group ,low-dose OXC group(100 mg/kg ,LDOXC) ,middle-dose OXC group(200 mg/kg ,MDOXC) and high-dose OXC group(300 mg/kg ,HDOXC) . The animal model of rats with epilepsy treated by different doses of OXC was established . After twenty-one days administration ,changes in behavioral performance were recorded ,and the NCAM protein level and mRNA in hippocampus were examined by immunohisto-chemisty and Real-time PCR respectively . Results Compared with PTZ group ,latent time and degree of epileptic seizures induced by PTZ were prolonged and alleviated in OXC groups (P<0 .01) ,the expression of NCAM in the astrocyte and NCAM mRNA in hippocampus were depressed in OXC groups (P<0 .05) . Conclusion NCAM may correlate with epilepsy closely .OXC could decrease the expression of NCAM with a dosage dependent manner .OXC may alleviate the brain damage caused by epilepsy .