苦荞麦蛋白质对乳腺癌细胞的作用机制

摘要

The mechanism of tartary buckwheat protein on breast cancer cells was studied by Flow cytometry (FCM).The inhibition effect of TBWSP31 on the proliferation of Bcap37 cell existed in dose- and time-dependent manners.After treatment with TBWSP31, Bcap37 cell resulted in the accumulation of the cell in G0/G1, and prevented the cell growing from G0/G1 phase to S phase.The cell cycle was related to G1 phase, and the longer G1 phase could slow the speed of cell malignant proliferation.The expression of bcl-2 and Fas were detected quantitatively by FCM.Based on the above results, it can be concluded that the antitumor mechanism was associated with the increasing expression of Fas and the decreasingd expression of bcl-2.%研究了苦养麦蛋白质TBWSP31对Bcap37乳腺癌细胞的作用机制.采用流式细胞仪分析测定苦养麦蛋白质TBWSP31对Bcap37细胞作用后,对细胞周期及癌基因和抑癌基因蛋白产生的影响.结果表明,TBWSP31对Bcap37细胞的增殖抑制作用存在时间效应和剂量效应.TBWSP31作用于Bcap37细胞后,G0/G1期细胞比例增加,DNA合成期-S期细胞减少,说明细胞受阻于G0/G1期,阻滞G0/G1期的细胞向S期转化,细胞的恶性增殖相应减慢.TBWSP31可以上调抑癌基因蛋白Fas的表达,下调癌基因蛋白bcl-2的表达.