高效液相色谱-串联质谱法同时测定婴幼儿配方食品中香兰素、甲基香兰素和乙基香兰素

摘要

Objective A method is presented for the quantitative determination and confirmation of vanil-lin, methyl vanillin and ethyl vanillin in infant formulas by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Methods Samples were extracted by Tris solution and acetonitrile. The supernatant was centrifuged and defatted byn-hexane before the 0.22μm filter apparatus was used, finally de-tected by HPLC/MS/MS and quantitated by external standard method.Results A good linearity of three compounds was obtained in the range of 5.0~200μg/L. The limits of detection of three compounds were 25μg/kg in liquid milk and 50μg/kg in milk powder. Mean recoveries of three compounds ranged from 89.5% to 115%. The relative standard deviation was less than 15.6%. Conclusion The method was proved to be a sim-ple, rapid, accurate and sensitive method and was successfully applied to detect vanillin, methyl vanillin and ethyl vanillin in infant formula.%目的：建立了高效液相色谱-串联质谱法同时测定婴幼儿食品中香兰素、甲基香兰素和乙基香兰素的方法。方法样品经 Tris 溶液和乙腈振荡提取,离心后提取液用正己烷脱脂过滤后上机测定,外标法定量。结果3种化合物在5.0~200μg/L范围内线性良好,在液态奶中测定低限均为25μg/kg,在奶粉中测定低限均为50μg/kg,方法加标收率在89.5%~115%之间,相对标准偏差(RSD)小于15.6%。结论该方法简便、快速,准确,灵敏度及精密度高,适用于婴幼儿食品中香兰素、甲基香兰素和乙基香兰素的测定。