粘红酵母CA815固态发酵废弃烟梗产油脂的Plackett-Burman优化

摘要

Objective To realize the resource utilization of waste tobacco stem (WTS) and obtain cheap fermentation substrate of microbial oil. Methods Rhodotorula glutinis CA815 was used as the starting strain, and solid-state fermentation of WTS was performed by shake flask method. After fermentation, a proper amount of normal saline was added and mixed evenly, and the biomass of Rhodotorula glutinis CA815 was obtained by double layer gauze filtration. The yeast cell lipid was extracted by acid-heating method. Plackett-Burman (PB) design was employed to evaluate some variables which were relevant to lipid production of Rhodotorula glutinis CA815 under solid-state fermentation of WTS. Results The PB design results showed that among eleven selected variables,peptone, yeast powder and culture time, as three key factors, were found to significantly rrninfluence lipid production,the highest biomass of Rhodotorula glutinis CA815 could reach 79.00 rrnmg/g, and the maximum production of lipid could reach 4.633 μg/g. Conclusion Under PB design rrnconditions, WTS is used as the main substrate of Rhodotorula glutinis CA815. By adding rrnappropriate amount of yeast powder, peptone and optimizing culture time,the lipid can be rrnproduced by solid -state fermentation from WTS.%目的 资源化利用废弃烟梗(waste tobacco stem,WTS)并获取微生物油脂的廉价发酵基质.方法 以一株粘红酵母(Rhodotorula glutinis)CA815为出发菌株,采用摇瓶方式固态发酵WTS,发酵后加入适量生理盐水并混合均匀,双层纱布过滤获得固态发酵的酵母生物量,采用酸热法提取酵母细胞油脂;采用Plackett-Burman(PB)实验设计法对影响微生物油脂产生的相关因素进行方差及贡献率分析.结果 PB试验结果统计学分析表明:在选择的11个相关因素中,酵母粉、蛋白胨和培养时间是影响酵母油脂产量的3个关键供试因素,粘红酵母CA815生物量最高值可达79.00 mg/g,油脂的最高产量可达4.633μg/g.结论 粘红酵母CA815以WTS为主要基质,通过添加适量酵母粉和蛋白胨、优化培养时间,既可资源化利用WTS,又可通过固态发酵的方式获得微生物油脂.