运用单标记分析法分析了10个微卫星位点与控制体重、体长数量性状的QTL的连锁关系.10对微卫星引物在8月龄具有生长性状差异的60个刺参个体基因组DNA中扩增得到50个等位基因,每个微卫星位点等位基因数3～7个,平均有效等位基因个数5.0.10个微卫星位点的多态信息含量为0.268 0～0.825 6不等,观测杂合度为0.083 3～0.666 7.采用SPSS软件对刺参的体重、体长生长性状和微卫星位点相关性进行分析,结果显示,微卫星位点AjSSR02基因型AB、AD、CD与体重和体长相关,AjSSR04标记中等位基因A对生长性状有正面影响,位点AjSSR06基因型FF个体仅平均体重与其他基因型个体显著差异,AjSSR09标记的A和B等位基因分别对生长性状具有正面和负面不同影响.%Ten microsatellite loci were selected to analyze the correlation with quantitative trait locus of body weight and length genomic of Apostichopus japonicus using single marker-based analysis. DNA of 60 eight months old individuals of Apostichopus japonicus which have differences in growth traits were analyzed by 10 microsatellite markers. The results showed that a total of 50 different alleles were found, the number of alleles in each locus was 3 to 7, and the number of mean valid alleles was 5. 0. The polymorphism information content(PIC) of ten microsatellite loci was 0.083 3 to 0.666 7, and the value of heterozygosity was 0. 236 1 to 0. 767 7, which proved the ten loci can be adopted to make correlation analysis. SPSS program was used to analyze the effects of these 10 microsatellites on body weight and length of Apostichopus japonicus. The results showed that AB, AD and CD genotypes of AjSSR02 had significant difference in body weight and length with other types, allele A of AjSSR04 played a positive role in both body weight and length, FF genotype of AjSSR06 had a significant impact on weight but not on length, and A and B allele of AjSSR09 played positive and negative roles in growth traits respectively.
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