L-酪氨酸分子印迹传感器敏感膜的制备与性能研究

摘要

以邻苯二胺（o-PD）为功能单体，采用电化学聚合的方法，在金电极表面电聚合成分子印迹聚合物膜。洗脱模板分子，优化制备过程的条件，获得了 L-酪氨酸（L-Tyrosine）分子印迹传感器敏感膜。并通过循环伏安法（CV)、示差脉冲伏安法（DPV)和电化学阻抗谱法（EIS）三种方法考察了电极的性能；在循环伏安法（CV）方法测试结果表明，模板分子 L-酪氨酸在磷酸缓冲溶液（PH=8.0）中与功能单体邻苯二胺能聚合并吸附在金电极表面，并且在聚合前后及洗脱模板分子前后峰电流有明显差异；由示差脉冲法（DPV）测试结果表明，在(1×10-2~2.0) mg/mL 范围内，峰电流与 L-酪氨酸的浓度成线性关系，检出为2.0 mg/mL。选择识别性实验结果表明，该分子印迹修饰电极对与 L-酪氨酸相似的 L-苯丙氨酸、L-丙氨酸、L-色氨酸、L-天冬氨酸的电流响应很小，说明分子印迹传感膜对 L-酪氨酸有特异性识别功能；EIS 方法测试表明，印迹电极对 L-酪氨酸分子具有识别作用。%L-Tyrosine as template molecule, o-phenylendiamine as functional monomer, on the gold electrode surface sensitive in situ synthesized molecularly imprinted polymer membranes. By cyclic voltammetry(CV), differential pulse voltammetry(DPV) and electrochemical impedance spectroscopy(EIS) to investigate the performance of the electrode. CV method tests show that the template molecules L-tyrosine in phosphate buffer solution (PH=8.0) o-PD and functional monomers to polymerization and adsorption on the gold electrode surface, and before and after polymerization and elution peak current have obvious difference before and after the template molecules. DPV test results show that in 1×10-2~2.0 mg/mL range, the peak current and a linear relationship with the concentration of L-tyrosine, the detection limit of 2.0 mg/mL. Chooseing the recognition experiment results show that the molecularly imprinted modified electrodes are similar to L-tyrosine L-phenylalanine, L-alanine, L-tryptophan, the current response of L-aspartate is very small, that molecularly imprinted sensor membrane have specific recognition of L-tyrosine.EIS method test showed that the imprinted electrode for L-tyrosine molecular recognition.