以解酯假丝酵母(Candida lipolytica)为出发菌株,采用摇瓶发酵法产酶,对发酵产的脂肪酶粗酶液进行了性质及固定化研究.该酶反应的最适温度为40℃,最适pH为7.5,在pH 6.0～8.0酶活较稳定,热稳定性较差.脂肪酶的最佳固定化条件为:载体硅藻土与脂肪酶质量比为14,固定化温度为30℃,缓冲液pH为8.0,固定化时间为2h.%The lipase produced by Candida lipolytica was characterized. The results show that the optimal temperature was 40℃ , the optimum pH was 7. 5. The enzyme was stable at pH 6. 0-8. 0 but did not have good thermal stability. The immobilized lipase was characterized and the optimum conditions were as follows: the mass ratio of carrier to enzyme was 14, and immobilizing rfor 2 h at 30℃and pH 8. 0.
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