营养胁迫调控弯曲隐球酵母转化N-乙酰-D-葡萄糖胺生产油脂

摘要

Cryptococcus curvatus ATCC 20509 using N-acetylglucosamine as unique carbon source was used to investigate phosphorus and sulfate limitation impact on lipid accumulation, and fatty acid compositions were also analyzed by GC-MS.The results illustrated that both phosphorus and sulfur limitation could regulate lipid accumulation under nitrogen-rich condition.The lipid content and yield increased with n(C)/n(P) ratio increasing, while, increased initially and then decreased with n (C)/n(S) ratio increasing.The fatty acid analysis showed that the content of saturated fatty acid decreased first and then increased with n(C)/n(P) ratio increasing, while the content of saturated fatty acid increased first and then decreased with n(C)/n(S) ratio increasing.%以一株可利用N-乙酰-D-葡萄糖胺(GlcNAc)为唯一碳源的菌株--弯曲隐球酵母(Cryptococcus curvatus ATCC 20509)为研究对象,分别采用磷元素限制和硫元素限制,考察对其油脂积累的影响,并对脂肪酸组分进行了分析.结果表明,磷、硫元素均可调控该酵母在氮源丰富条件下的油脂积累,油脂含量和油脂得率随碳磷摩尔比的增高而增高,随碳硫摩尔比的增高呈先上升后下降的趋势.脂肪酸组分分析表明,饱和脂肪酸含量随着碳磷摩尔比的增加先下降后上升,随着碳硫摩尔比的增加呈先上升后下降的趋势.