氢氧化钙对粪肠球菌生物膜形成相关毒力因子作用的研究

摘要

目的：研究粪肠球菌毒力因子gelE、cylA、ace、esp在氢氧化钙作用粪肠球菌生物膜后的表达情况。方法体外建立对数期、稳定期、饥饿期粪肠球菌生物膜模型，以氢氧化钙作用于粪肠球菌生物膜后用qRT－PCR检测毒力因子gelE、cylA、ace、esp的基因表达相对量。结果氢氧化钙作用后，gelE、cylA、ace、esp基因表达相对量，饥饿期高于稳定期及对数期；饥饿期gelE基因表达相对量高于esp，也高于cylA及ace，差异具有显著性意义（ P＜0．05）。结论氢氧化钙作用生物膜可抑制粪肠球菌毒力因子gelE、cylA、ace、esp的表达。%Objective To research the virulence factor expressions of enterococcus faecalis in the biofilm after Ca( OH) 2 . Methods Real-time quantitative polymerase chain reaction ( qPCR) was used for detecting the expressions of gelE,ace, esp, cylA in exponential phase, log phase and starvation phases after Ca( OH) 2 .Results Treated state, the expressions of gelE, ace, esp, cylA of the enterococcus faecalis biofilm were stronger in starvation phase than in exponential phase and log phase (P<0.05).Treated state, the expression of gelE is stronger in starvation phase than ace, esp, cylA (P<0.05). Conclusion The Ca( OH) 2 plays an important role in inhibition of biofilm formation.