Objective To analyze the effects of different doses of musk and borneol on neurological deficit score,content of 5-lipoxygenase (5-LOX)and cyclooxygenase 2(COX-2)and CysLT2 expression in hippocampus of cerebral ischemia-reperfusion (IR)model. Methods 50 male SD rats were randomly divided into low-dose of musk group (25 mg/kg),high-dose of musk group (50 mg/kg)and low-dose of borneol group (25 mg/kg)and high-dose of borneol group (50 mg/kg)and model group (the 1％ volume Twain solution),with 10 rats in each group. The differences of neurological deficit scores,5-LOX and COX-2 contents in brain tissue and expression of CysLT2 protein in hippocampus of 1 and 3 days after cerebral ischemia in different groups were compared. Results In 3 days after cerebral ischemia,the neurological deficit scores of the low-dose od musk group,low-dose group and high-dose of borneol group were significantly higher than those in 1 day (P<0.05). At 1 day after cerebral ischemia,the 5 -LOX content of musk group with different dose and low-dose of borneol group were significantly lower than that of the model group (P<0.05). In 3 days,the 5-LOX content of high-dose in musk and borneol group were significantly lower than that of the model group (P<0.05). In 3 days,the content of 5-LOX in the low-dose group of musk and borneol group were significantly higher than that of 1 day (P<0.05). In 3 days after cerebral ischemia,the content of COX-2 in different doses of musk and borneol group were significantly higher than those in 1 day (P<0.05). At 1 day after cerebral ischemia,the expression level of CysLT2 protein in hippocampus of the high-dose borneol group was significantly lower than that in the model group (P<0.05). At 3 days,the expression level of CysLT2 protein in hippocampus of high-dose musk and borneol group were significantly higher than those in the model group (P<0.05). Conclusion Musk and borneol can effectively improve pathological morphology of brain tissue and neurological deficits in IRI rats,reduce 5-LOX and COX-2 activities in brain tissue and inhibit the ex-pression level of CysLT2 protein in the hippocampus,which suggesting that the musk and borneol can inhibit the inflammatory injury in IR rats,and the mechanism may be closely related to inhibit the activities of 5-LOX and COX-2 in brain tissue.%目的 分析不同剂量麝香、冰片对脑缺血-再灌注(IR)模型神经功能缺损评分和脑组织5-脂氧酶(5-LOX)和环氧酶2(COX-2)含量及海马CysLT2表达的影响.方法 选取50只雄性SD大鼠,随机分为麝香低剂量组(25mg/kg)、麝香高剂量组(50mg/kg)、冰片低剂量组(25mg/kg)、冰片高剂量组(50mg/kg)及模型组(采用等体积1％吐温溶液),各组均为10只.各药物组均于建模前灌胃给予相应药物,模型组给予等量生理盐水.分别比较不同分组大鼠脑缺血1d、3d时神经功能缺损评分和脑组织5-LOX、COX-2含量及海马组织CysLT2蛋白表达水平的差异.结果 脑缺血3d时,麝香低剂量组和冰片高、低剂量组神经功能缺损评分较1d时均显著升高(P<0.05).脑缺血1d时,不同剂量的麝香组和冰片低剂量组5-LOX含量均明显低于模型组(P<0.05);3d时麝香、冰片高剂量组5-LOX含量明显低于模型组(P<0.05).3d时麝香、冰片低剂量组5-LOX含量较1d时均显著升高(P<0.05).脑缺血3d时不同剂量的麝香、冰片组脑组织COX-2含量较1d时均明显升高(P<0.05).脑缺血1d时冰片高剂量组海马组织CysLT2蛋白表达水平较模型组明显降低(P<0.05),3d时麝香、冰片高剂量组海马组织CysLT2蛋白表达水平较模型组均明显升高(P<0.05).结论 麝香、冰片可有效改善IRI大鼠脑组织病理形态和神经功能缺损程度,减轻脑组织5-LOX、COX-2活性,阻滞海马组织CysLT2蛋白的表达水平,提示二者具有抑制大鼠IR炎性损伤的作用,其作用机制可能与阻滞脑组织5-LOX、COX-2活性密切相关.
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