系统性硬化症和混合性结缔组织病活动期血清中LOXL-2的比较

摘要

Objective To investigate the expression of lysyl oxidase like protein⁃2(LOXL⁃2)in the sera of patients with active systemic scleroder⁃ma(SSc)and mixed connective tissue disease(MCTD). Methods An enzyme⁃linked immunosorbent assay was adopted to measure LOXL⁃2 in the serum of 20 patients with active SSc,20 patients with active MCTD,and 20 healthy controls. The measurements among different groups was com⁃pared,and correlations between LOXL⁃2 levels and clinical manifestations of SSc and MCTD were examined. Results The levels of LOXL⁃2 ex⁃pression in MCTD and SSc groups were significantly higher than those in control group(P<0.05 for all groups). LOXL⁃2 expression is also related to the presence of skin lesions in SSc(r=0.982 P=0.001). Conclusion High serum level of LOXL⁃2 in these patients with active SSc and active MCTD suggests that LOXL⁃2 may be involved in the process of fibrosis and the resulting vasculitis in multiple organs.%目的：比较赖氨酰氧化酶样蛋白2（LOXL⁃2）在系统性硬化症（SSc ）和混合性结缔组织病（MCTD ）活动期患者血清中的表达情况。方法用酶联免疫吸附试验（ELISA）法检测20例SSc和20例MCTD患者中LOXL⁃2的浓度，进行统计分析。结果在活动期MCTD和SSc患者血清中LOXL⁃2的蛋白表达量高于对照组（均P<0.05），且在SSc患者中LOXL⁃2浓度与患者皮肤病变评分（mRSS）呈正相关（r=0.982 P=0.001）。结论 LOXL⁃2在SSc和MCTD活动期患者的血清中高表达，可能与LOXL⁃2促进组织纤维化及血管炎的发生相关。