白介素4、10、12、13、IFN-γ、TGF-β在不同时期特应性皮炎病人血清中的变化

摘要

目的 研究疾病发作期和缓解期特应性皮炎(atopic dermatitis,AD)病人血清中白细胞介素-4(interleukin-4,IL-4)、IL-10、IL-12、IL-13、干扰素-γ(interferon-γ,IFN-γ)、转化生长因子-β(transforming growth factor-β,TGF-β)等细胞因子的变化,探讨其在AD发病机制中的作用.方法 收集疾病发作期AD病人79例,其中经治疗皮损缓解的AD病人(缓解组)40例,未缓解的AD病人(未缓解组)39例.采用酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)测定病人治疗前后血清中IL-4、IL-10、IL-12、IL-13、IFN-γ、TGF-β的浓度.结果 缓解组AD病人治疗后血清中IL-4、IL-12、IL-13、IFN-y与治疗前相比,差异均有统计学意义(均P＜0.05).缓解组AD病人治疗后血清中IL-10、TGF-β与治疗前相比,差异无统计学意义(P＞0.05).未缓解组AD病人治疗后血清中IL-4、IL-10、IL-12、IL-13、IFN-γ、TGF-β与治疗前相比,差异均无统计学意义(P＞0.05).结论 AD病人病情缓解时血清中IL-4、IL-13表达显著下调,IFN-γ和IL-12表达显著升高.%Objective To investigate the changes of interleukin-4(IL-4),IL-10,IL-12,IL-13,interferon-γ (IFN-γ) and transforming growth factor-β (TGF-β) in the serum of atopic dermatitis patients at different stages of the disease,and explore their significance.Methods Seventy-nine atopic dermatitis patients at acute stage were enrolled.There were 40 cases at remission stage(remission group,RG),and 39 cases were still at acute stage after treatment(acute group,AG).The serum levels of IL-4,IL-10,IL-12,IL-13,IFN-γ and TGF-β were detected by enzyme-linked immunosorbent assay (ELISA) before and after treatment.Results There were significant differences in the serum levels of IL-4,IL-12,IL-13 and IFN-γ before and after treatment in the remission group(P ＜0.05).There were no significant differences in the serum levels of IL-10,TGF-β before and after treatment in the remission group(P ＞ 0.05).There were no significant differences in the serum levels of IL-4,IL-10,IL-12,IL-13,IFN-γ and TGF-β before and after treatment in the acute group(P ＞ 0.05).Conclusion Lower expression of IL-4,IL-13,and higher expression of IFN-γ,IL-12 in serum at remission stage of atopic dermatitis.