目的 检测脑小血管病 (CSVD) 患者血清中炎症小体相关炎症因子IL-1β、IL-18浓度, 建立炎症小体NLRP3与CSVD之间的潜在关联.方法 纳入CSVD患者50例和同期具有脑血管病危险因素, 但头颅影像学检查正常的患者 (主要指头颅磁共振平扫及弥散成像) 30例.采集血样标本, 使用双抗体夹心法对血清IL-1β、IL-18浓度进行检测, 比较其与CSVD的相关性.结果 CSVD组IL-1β血清浓度 (19.21±3.77ng·L-1) 比对照组 (16.56±2.84ng·L-1) 高 (P<0.05) ;CSVD组IL-18血清浓度 (73.38±19.27ng·L-1) 比对照组 (70.70±2.84ng·L-1) 高, 但差异无统计学意义 (P>0.05) .以CSVD为因变量, 以年龄、性别、高血压、糖尿病、冠心病、既往卒中史、吸烟史、饮酒史、颈动脉内膜增厚、颈动脉斑块形成、血同型半胱氨酸、糖化血红蛋白、空腹血糖、三酰甘油、低密度脂蛋白、总胆固醇、IL-1β、IL-18为自变量, 进行Logistic回归分析.IL-1β、年龄、吸烟是CSVD的危险因素 (B=0.446, OR=1.562, P=0.003;B=0.166, OR=1.180, P=0.007;B=4.107, OR=0.016, P=0.010) .结论 血清IL-1β浓度升高与CSVD有相关性;IL-1β、年龄、吸烟是脑小血管病的危险因素.%Objective To investigate the potential association between NLRP3 inflammasome and cerebral small vessel disease (CSVD) by detecting the concentrations of NLRP3-related inflammatory factors IL-1β and IL-18 in CSVD patients. Method Fifty patients diagnosed with CSVD and collected 30 cases with cerebrovascular disease risk factors, however, the skull imaging examination of the normal patients (mainly refers to the head of the nuclear magnetic flat sweep and dispersion) . The blood specimens were collected, and the concentrations of serum IL-1β and IL-18 were detected by using double antibody sandwich method, and their correlations with CSVD were compared. Results The serum concentration of IL-1β in CSVD group was higher (19.21±3.77 ng·L-1) than the control group (16.56±2.84 ng·L-1) , showing statistically significant difference (P<0.05) . Similarly, the serum concentration of IL-18 in CSVD group was higher (73.38±19.27 ng·L-1) than the control group (70.70±2.84 ng·L-1) , but showed no significance. Logistic regression analysis was carried out by using CSVD as dependent variable, and age, gender, hypertension, diabetes, coronary heart disease, previous history of stroke, smoking history, drinking history, carotid artery intima thickening, carotid artery plaque formation, serum homocysteine, glycosylated hemoglobin, fasting venous blood glucose, triglycerides, low density lipoprotein, total cholesterol, IL-1β, IL-18 as independent variables.The results showed that IL-1β, age and smoking were the risk factors for CSVD (B=0.446, OR=1.562, P=0.003;B=0.166, OR=1.180, P=0.007; and B=4.107, OR=0.016, P=0.010) . Conclusion These findings suggested a correlation between the concentrations of serum IL-1β and CSVD. Also IL-1β, age and smoking are considered as the risk factors for CSVD.
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