基质辅助激光解吸/电离飞行时间质谱在鼠伤寒沙门菌同源性分析中的应用价值

摘要

Objective:To investigate the value of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry( MALDI-TOF MS) in S. typhimurium homology analysis. Methods:Forty-four S. typhimuium strains isolated from Guangzhou were typed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, which was compared with pulse-field gel electrophoresis ( PFGE) to analyze the main patterns of mass spectrogram. Results:The PFGE results showed that 44 S. typhimuium strains could be divided into 15 types. At 80% of the clustering similarity,44 S. typhimuium strains could be divided into 8 MALDI-TOF MS types, which was accordance to the PFGE results. The MS1 ( 52. 3%) was the main type, which contained multiple PFGE patterns. Conclusions:The classification results of MALDI-TOF MS show the relative concentration of kinship of S. typhimuium isolated from Guangzhou,which is a simple,rapid,high throughput method of classification.%目的:研究基质辅助激光解吸/电离飞行时间质谱(MALDI-TOF MS)在鼠伤寒沙门菌同源性分析中的应用价值.方法:利用MALDI-TOF MS对分离于广州市的44株鼠伤寒沙门菌进行分型,与脉冲场凝胶电泳分型(PFGE)进行比较,并分析主要质谱型的质谱图.结果:对PFGE结果聚类分析可以将44株鼠伤寒沙门菌分为15型.在聚类相似度为80%时,44株鼠伤寒沙门菌被分为8个MALDI-TOF MS型,与PFGE分型较一致.其中MS1型(52.3%)为主要的质谱型,MS1型包含多个PFGE型.结论:MALDI-TOF MS方法分型结果显示了广州市鼠伤寒沙门菌相对集中的亲缘关系,是一种简便、快速、高通量的方法.