首页> 外文会议>International Symposium on Runaway Reactions, Pressure Relief Design and Effluent Handling American Institute of Chemical Engineers/American Chemical Society Management Conference >Identification of Proteins in Unsequenced Bacterial Strains Via Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Identification of Proteins in Unsequenced Bacterial Strains Via Matrix-Assisted Laser Desorption/Ionization Mass Spectromet
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Identification of Proteins in Unsequenced Bacterial Strains Via Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Identification of Proteins in Unsequenced Bacterial Strains Via Matrix-Assisted Laser Desorption/Ionization Mass Spectromet

机译:通过基质辅助激光解吸/电离质谱法通过基质辅助激光解吸/电离质谱鉴定通过基质辅助激光解吸/电离质谱鉴定蛋白质中的蛋白质中蛋白质的鉴定

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摘要

This work presents a protocol that has been developed to identify proteins from bacterial strains for which no DNA or protein sequence exists. A particular set of proteins are characterized using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) to obtain accurate protein molecular weights. The resulting molecular weights are then compared to DNA or protein sequences from reference strains, allowing for straightforward identification of proteins from any non-reference strains. This approach allows for the facile determination of differences in proteins at the amino-acid level as well as the post-translational level. This protocol was developed, validated, and applied to ribosomal proteins from various strains of Thermus thermophilus. The protocol allows for rapid identification of proteins and will be useful in phylogenic studies and biomarker identification.
机译:该工作提出了一种协议,已经开发出用于鉴定来自没有DNA或蛋白质序列的细菌菌株的蛋白质。特定的蛋白质是使用基质辅助激光解吸/电离飞行时间质谱(MALDI-TOFM)的特征,以获得精确的蛋白质分子量。然后将得到的分子量与来自参考菌株的DNA或蛋白质序列进行比较,从而允许从任何非参考菌株中直接鉴定蛋白质。这种方法允许体测定氨基酸水平和翻译后水平的蛋白质中的差异。开发,验证该方案,并应用于来自各种热嗜热菌株的核糖体蛋白。该方案允许快速鉴定蛋白质,可用于系统发育研究和生物标志物鉴定。

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