尿培养产ESBLs大肠埃希菌的耐药特点及对磷霉素的敏感性分析

摘要

目的：：分析尿培养产超广谱β内酰胺酶( ESBLs)大肠埃希菌的耐药特点,了解磷霉素对产ESBLs大肠埃希菌的体外抗菌活性,并与其他种抗菌药物进行比较。方法：从菌株库复苏2010-2014年尿培养大肠埃希菌821株,使用ESBLs的表型确认实验筛选产ESBLs菌株,用K-B法测定磷霉素等15种抗菌药物对其的体外抗菌活性。结果：821株大肠埃希菌中465株产ESBLs,对亚胺培南敏感性较高(91.4%),其次是磷霉素(88.0%),然而对左氧氟沙星、青霉素类和头孢菌素类抗生素的敏感性较差。结论：碳青霉烯类抗生素依然是敏感率最高的药物,磷霉素对尿培养产ESBLs大肠埃希菌有良好的体外抗菌活性,具有一定的临床应用价值。%Objective:To analyze the resistant characteristics of ESBLs Escherichia coli produced by urine culture, investigate its antibacterial activity in vitro and compare with other antibacterial drugs. Methods:A total of 821 strains were cultured from the strain library,the ESBLs Escherichia coli produced by urine culture were screened according to the phenotype of ESBLs, the antibacterial activities in vitro of 15 antimicrobial agents,including fosfomycin,were detected by K-B method. Results:Among the 821 Escherichia coli strains,the sensitivities of the 465 ESBLs Escherichia coli strains produced by urine culture to imipenem were high(91. 4%), following by fosfomycin(88. 0%),the sensitivities of which to levofloxacin,penicillin and cephalosporin were low. Conclusions:The sensitivity of carbapenem is the highest drug,the antibacterial activity in vitro of fosfomycin to ESBLs Escherichia coli produced by urine culture is good,which has certain clinical value.