[Objective] The aim was to study the process condition of preparing the ferulic acid by wheat bran through enzyme method and alkali extraction method. [Method] The wheat bran was pretreated by using enzyme to remove the starch, protein and sugar material. The ferulic acid was extracted by using sodium hydroxide and the proportion of bran and lye was 1∶20. The optimum process conditions for treating the dry wheat bran by enzyme method and extracting the ferulic acid by lye were confirmed resp. through orthogonal test. [Result] The optimum process condition for pretreating the dry wheat bran with enzyme method was that the enzyme dosage of high-temperature resistant α-amylase, protease and glucoamylase were 2 000, 1 398 and 50 000 U/g bran resp. The optimum process condition for extracting the ferulic acid from the enzyme-pretreated bran by using alkali method was that the sodium hydroxide concn. was 1.5%, the extraction temperature was 85 ℃, the extraction time was 4 h. Adding 0.5% KBH4 into the sodium hydroxide could effectively increase the retained dose of ferulic acid in the extract liquid and the yield of ferulic acid was up to 9.414%-10.937%. [Conclusion] This research provided the scientific basis for increasing the utilization ratio and added value of wheat bran.%[目的]研究通过酶法和碱提取方法利用小麦麦麸制备阿魏酸的工艺条件.[方法]用酶对小麦麦麸进行预处理,去除淀粉、蛋白质和糖类物质,用氢氧化钠提取阿魏酸,麦麸与碱液的比例为1∶20,通过正交试验分别确定酶法处理干燥小麦麦麸和碱液提取阿魏酸的最优工艺条件.[结果]用酶法预处理干燥小麦麦麸的最优工艺条件是:耐高温α-淀粉酶、蛋白酶和糖化酶的加酶量分别为2 000、1 398和50 000 U/g麦麸;用碱法从经酶预处理的麦麸中提取阿魏酸的最佳工艺条件是:氢氧化钠浓度为1.5%,提取温度为85 ℃,提取时间4 h,同时在氢氧化钠中加入0.5%的KBH4,可有效增加阿魏酸的在提取液中的保留量,阿魏酸得率可达9.414%～10.937%.[结论]该研究为提高小麦麦麸的利用率和附加值提供科学依据.
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