[ Objective ] The purpose of the research was to supply theoretical foundation for further exploitation of Aureobasidium pullulans.[ Method] A strain ofA. pullulans was isolated from soil by plate-dilution method, its mycelium was cultured with YDP medium and the microshape of its mycelium was observed through microimaging. Then the inner transcription section (ITS) of its rRNA gene was cloned and sequenced by molecular biological method and the sequencing result was compared with the known sequence in Genbank. [ Result ] The sequencing result of PCR amplification product from ITS showed that the length of amplified sequence was 606 bp, the isegenesis rate between it and Napicladium in Genbank was 98％ -99％ and it belongs to the same individual branch with A. pullulans wb 149 and A. pullulans HK58-1 (2); the results from morphological and molecular identification showed that the culture of A. pullulans was successful. [ Conclusion] The classic method and modem molecular technology was combined in this research, then a strain of A. pullulans was identified and this research supplied basis for category judgement of A. pullulans.%[目的]为出芽短梗霉的进一步开发利用提供理论依据.[方法]采用平板稀释法从土壤中分离出1株出芽短梗霉,用YPD培养基培养菌丝体,显微成像获得菌丝微观形态,利用分子生物学方法对其rRNA基因内转录区段(ITS 区)进行克隆测序,并与Genbank中已知序列进行比较.[结果]ITS区PCR扩增产物测序结果表明,所扩增序列的长度为606 bp,与Genbank中短梗霉的同源率为98%～99%,与Aureobasidium pullulans wb 149、A.pullulans HK58-1(2)属于同一单独分枝;形态及分子鉴定结果表明,出芽短梗霉培养成功.[结论]该研究采用经典与现代分子技术相结合的方法鉴定出了1株出芽短梗霉,为出芽短梗霉的分类鉴定提供了依据.
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