山药多糖提取工艺的优化及抗氧化活性的测定

摘要

[目的]优化山药多糖的提取工艺,并测定其抗氧化活性.[方法]在单因素试验的基础上,用正交试验优化山药多糖的提取工艺,并对不同蛋白质结合程度的山药多糖羟自由基清除率进行测定.[结果]山药多糖提取的最佳工艺参数为:提取温度为60℃,提取时间为3.0 h,料液比为1:8,pH值为8,在最佳工艺条件下,山药多糖的平均提取率为15.1%;经蛋白酶水解脱蛋白后的山药多糖抗氧化活性最高,其次为Sevage法脱蛋白处理后的山药多糖,而未经处理的山药多糖液抗氧化活性最低.[结论]该研究优化了山药多糖的提取工艺,为山药多糖的开发提供了技术支持.%[ Objective ] To optimize the extraction parameters of RHIZOMA DIOSCOREAE polysaccharide, and determine its antioxidant activity. [ Method ] The extraction process was optimized with orthogonal test based on single factor test, and the hydroxyl radical scavenging rate of DIOSCOREAE RHIZOMA polysaccharide with different degree of protein binding was comparatively determined. [ Result] The optimum extraction parameters were: extraction temperature of 60℃, extraction duration of 3.0 h, solid- to- liquid ratio of 1:8 and pH value of 8. Under the optimal conditions the mean extraction rate of RHIZOMA DIOSCOREAE polysaccharide was 15.1％. The antioxidant activity of RHIZOMA DIOSCOREAE polysaccharide hydrolyzed with protease, treated with Sevage method and untreated ranked the first, second and last, respectively.[ Conclusion ] The optimal extraction parameters are gained in this study, which provides technical support for the development of RHLZOMA DIOSCOREAE polysaccharide.