培养基营养条件对大豆根腐病病原菌菌丝生长和孢子形成的影响

摘要

[ Objective ] The aim was to explore effects of medium nutrients on mycelial growth and conidial production of soybean root rot pathogens in Anhui province. [Method] Effects of different solid mediums, carbon sources and nitrogen sources on the mycelial growth and conidial production of Fusarium solani which was the main pathogen of soybean root rot in Anhui province were investigated. [ Result ] The better media for mycelial growth of F. solani was PDA media among the 7 tested media. The optimum medium for conidial production was Cza-pek medium. For mycelial growth and conidial production on solid media, the optimum carbon source was sucrose. The optimum nitrogen sources for mycelial growth was urea, and the optimum nitrogen sources for conidial production was NH4NO3. [Conclusion] The research provides theoretical basis for relative study on F. solani and comprehensive treatnent of caused soybean root rot.%[目的]探讨培养基营养条件对安徽省大豆根腐病病原菌菌丝生长和孢子形成的影响.[方法]研究了不同培养基、碳源、氮源对安徽地区大豆根腐病主要致病菌——茄病镰孢(Fusarium solani)生长速率及产孢量的影响.[结果]在供试的7种培养基中,该菌在PDA培养基上生长最好,产孢量在查彼培养基上最高；该菌在以蔗糖为碳源的培养基上生长最快,产孢量最高;在以尿素为氮源的培养基上生长最快,产孢量在以硝酸铵为氮源的培养基上最高.[结论]为该菌的相关研究以及所致大豆根腐病的综合防治提供了理论依据.