喷雾干燥法中药益生元微胶囊的制备及体外控释分析

摘要

[目的]寻找水产养殖中抗生素替代品。[方法]以优化的中药配方与相应的肠道益生菌群合理配比形成益生元产品，采用喷雾干燥工艺制备中药复合益生菌群微胶囊制剂。对中药益生元微胶囊中益生菌存活率有显著影响的进风温度、出风口温度和进料速度3个因素为自变量，以微胶囊中益生菌的存活率为响应值，设计3因素3水平响应面分析试验，通过响应面分析确定微胶囊包埋的最佳条件，并通过体外模拟进行消化性能分析。[结果]微胶囊包埋的最佳条件为进风口温度187℃、出风口温度60℃和进料速度为32 mL／min，在此喷雾干燥工艺条件下中药益生元微胶囊中益生菌存活率达到9．42 logCFU／g，最终的微胶囊产品经SEM观察颗粒外形圆整，大小分布均匀且表面光滑。体外释放结果表明，壁材为质量分数1％的海藻酸钠和质量分数3％的明胶，在此工艺条件下制备的中药益生元微胶囊避免其在斜带石斑鱼胃液中快速吸收，减缓其在肠液中释放，确实提高中药益生元微胶囊的靶向性和高效性，进一步体外攻毒试验及H．E染色组织病理切片可观察到微胶囊对于鳗弧菌感染的鱼体受损肝脏器官具有明显疗效。[结论]制备的中药益生元微胶囊在体外模拟试验中具有较好的高效性和可控释放性能，该研究结果可为该微胶囊的市场化推广奠定基础。%Objective] To find the substitutes for antibiotics in aquaculture.[Method] On the basis of the synergistic effect of probiotics and optimal ingredient pattern of Chinese herbal medicine as the prebiotic products, we used spray drying technology to prepare microcapsule of probiotics and Chinese Herbal medicine.Three factors having significant impacts on the survival rate probiotics in microcapsule were used as the independent variables, which were air inlet temperature, air outlet temperature and feed speed.With survival rate probiotics in microcap-sule as the response value, response surface analysis experiment was designed with three factors and three levels.The optimal condition for mi-crocapsule embedding was obtained by response surface analysis.Digestive performance was analyzed by in vitro simulation.[ Result] The op-timal condition for microcapsule embedding was 187 ℃ air inlet temperature, 60 ℃ air outlet temperature and 32 mL/min feed speed.Under this condition, survival rate of probiotics in microcapsule reached 9.42 logCFU/g.Scanning Electron Microscopy (SEM) was applied to ob-serve the particle shape, which was even, smooth and round.The in vitro drug release test showed that the wall material was 1% alginate and 3% gelatin.Under this technology, the prepared microcapsule could avoid the fast absorption in gastrin of Epine phelus coioides, slowed down the release in intestinal juice, and enhanced the targeting and efficiency of microcapsule.The virus challenge in vitro test and H.E staining tis-sue pathological slices showed that the microcapsule had obvious curative effects on the fish liver damage infected by Vibrio anguillarum. [Conclusion] The prepared microcapsule has relatively high efficiency and controlled release performance during in vitro test.This research results lays foundation for the market promotion of this microcapsule.