败血症新生儿外周血细胞免疫变化规律研究

摘要

Objective To analyze the change of peripheral-blood lymphocyte in infants with neonatal septicemia and explore the role of peripheral-blood lymphocyte in the progression of neonatal septicemia in infants.Methods This was a retrospective case-control study conducted from June 2015 to September 2017.One hundred and thirty-two infants with neonatal septicemia were enrolled into this study,and 46 normal infants for a control group.Peripheral blood was drawn for the analysis of lymphocytes and complements and the data were statistically analyzed and compared between these groups.Results The CD4+ T lymphocytes and NK cells in the infants with neonatal septicemia significantly decreased.The decrease of CD4+ T lymphocytes and NK cells in the infants with early-onset septicemia,compared with that in the infants with late-onset septicemia,was more significant.Compared with those in the infants with early-onset septicemia and the control group,the depletion of C3 in the infants with late-onset septicemia was more significant.Conclusions CD4+ T lymphocytes,NK cells,complement C3 significantly decrease in infants with neonatal septicemia.%目的 研究新生儿败血症患儿外周血淋巴细胞亚群和补体的变化及其与不同类型新生儿败血症的关系;方法 本实验为回顾性病例对照试验,132例本院NICU住院的新生儿败血症患儿为研究对象,同时期同胎龄健康新生儿为对照组.研究对象于入院后当天抽取外周血,以流式细胞技术测量外周血T淋巴细胞、B淋巴细胞、NK细胞,并测定血清补体C3、补体C4,测量结果进行组间比较;结果 (1)新生儿败血症患儿外周血CD4+T淋巴细胞和NK细胞有明显下降,差异有统计学意义(P=0.019);(2)相对于迟发型败血症,早发型败血症外周血CD4+T淋巴细胞和NK细胞下降更明显,差异有统计学意义(P=0.038,P=0.0 15);(3)与迟发型败血症比较,早发型败血症患儿外周血补体C4有明显下降,差异有统计学意义(P=0.031);结论 新生儿败血症患儿外周血淋巴细胞有规律性变化,CD4+T淋巴细胞、NK细胞和补体C4作为免疫学指标对新生儿败血症有诊断意义.