目的：研究5，10‐亚甲基四氢叶酸还原酶（MTHFR）基因C677T位点突变，探讨其与孕妇异常高凝状态的关系。方法采用聚合酶链式反应‐熔解曲线分析法，检测50例异常高凝状态孕产妇和44例健康对照孕产妇的MTHFR基因C677T位点突变情况。结果异常高凝组和对照组MTHFR基因C677T3种基因型在两组的总体分布差异有统计学意义（P＝0．033），其中高凝组CC基因型表达频率明显降低（ P＝0．01 ），T等位基因表达频率明显增大（ P＝0．005）。结论孕产妇异常高凝状态与M T HFR基因C677T位点突变有关。%Objective To investigate methylenetetrahydrofolate reductase (MTHFR) C677T in pregnant woman with hyperco‐agulable state .Methods Polymerase chain reaction‐melting curve was employed to detect the mutation of MTHFR C677T in 50 hy‐percoagulable pregnant woman and 44 normal controls .Results The frequencies of the three C677T associated genotypes distribu‐ted between hypercoagulable pregnant woman and control group reached the statistical significance as a whole (P=0 .033) .The fre‐quency of genotype CC was significantly decreased (P=0 .01) ,and allele T was significantly increased (P=0 .005) in hypercoagu‐lable pregnant woman .Conclusion The mutation of MTHFR C677T plays a role in the mechanism of hypercoagulable pregnant woman in Chinese population .
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