目的：研究间接免疫荧光法（IIF）筛查的抗核抗体（ANA）和免疫印迹法（IBT ）检测的特异性ANA 谱在系统性红斑狼疮（SLE）患者的相关性和临床意义。方法对80例SLE患者（SLE组）、80例其他风湿病患者（疾病对照组）及80例健康体检者（健康对照组）分别采用IIF、IBT检测血清中的ANA。结果 SLE组ANA阳性率（96．25％）显著高于疾病对照组（32．50％）和健康对照组（5．00％），差异有统计学意义（P＜0．05）；SLE组荧光模式主要是颗粒型（43例，53．75％）、均质型（16例，20．00％）、胞浆颗粒型（14例，17．50％）。SLE组ANA的阳性率为97．5％，其中抗nRNP／Sm抗体、抗Sm抗体、抗dsDNA抗体、抗核小体抗体、抗组蛋白抗体、抗Rib‐P抗体阳性率均显著高于疾病对照组和健康对照组，差异有统计学意义（P＜0．05）。结论 ANA和ANA谱联合检测对SLE的诊断及治疗具有重要意义。%Objective To discuss the relationship and clinical significance of the indirect immunofluorescence(IIF) assay screen‐ing for antinuclear antibody(ANA) and Immunoblotting test(IBT) for antinuclear antibody spectrum(ANAs) .Methods The ser‐um ANA level and other autoantibodies levels in ANAs of 80 patients with SLE(SLE group) ,80 patients with other rheumatic dis‐eases(disease control group) and 80 healthy people(control group) were detected with indirect immunofluorescence and immunob‐lotting test respectively .Results The positive rate of ANA in SLE group(96 .25% ) was significantly higher than that in disease control(32 .50% ) and control group(5 .00% ) ,differences were statistically significant(P< 0 .05);The major immunofluorescent pattern was 3 styles ,43 cases of granuliferous(53 .75% ) ,16 cases of homogeneous(20 .00% ) ,14 cases of cell plasma particles (17 .50% ) .The positive rate of ANAs in SLE group was 97 .50% .Compared with disease control and control group the positive rate of anti‐nRNP/Sm antibody ,anti‐Sm antibody ,anti‐dsDNA antibody ,antinucleosome antibody ,anti‐histone antibody and anti‐ri‐bosomal P protein antibody in ANAs of SLE group were significant higher ,differences were statistically significant(P<0 .05) .Con‐clusion The combined detection of ANA and ANAs have important significance in the diagnosis and treatment of SLE .
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