利用水浸提法提取茶树菇(A grocybe cylindracea)多糖,通过单因素试验考察料水质量比、浸提时间、pH、醇析时乙醇用量等4个因素对茶树菇粗多糖得率的影响.结果表明,茶树菇粗多糖的最佳提取条件为料水质量比1:10、浸提时间2h、pH 7.5、浸提浓缩液4倍体积的95％乙醇醇析.用乙醚和Sevage法纯化茶树菇多糖,并对其抗氧化性能进行了分析,发现茶树菇多糖具有良好的抗氧化性能.在试验浓度范围内,其总还原能力和对羟自由基、超氧阴离子自由基、脂过氧自由基的清除能力随多糖浓度的升高而增强,且其在酸性条件下对亚硝酸根离子具有良好的清除能力.%Polysaccharide was extracted from Agrocybe cylindracea using water as solvent. According to single factor tests, conditions for obtaining high polysaccharide yield were, mass ratio of material to water, l:10; Extraction time, 2 h; pH, 7.5 and volume ratio of 95% ethanol to concentrated extract solution for alcohol precipitation, 4:1. The extracted crude polysaccharide was refined by ether and Sevage reagent and its antioxidant activity was determined. The results showed that polysaccharide of A. Cylindracea was with great antioxidant activity, ability of clearing hydroxyl radical, superoxide anion radical, and lipid peroxy radical nitrite ion increased with the increase of polysaccharide dosage in the scope of test concentration; And it also had excellent clearing ability to nitrite ion in acidity condition.
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