响应面法优化虎斑乌贼内脏多糖提取工艺及抗氧化活性、吸湿保湿性能研究

摘要

本文以虎斑乌贼内脏为原料,采用Box-Benhken设计优化盐法提取多糖工艺,并对其体外抗氧化活性和吸湿-保湿性能进行研究.结果表明:虎斑乌贼内脏多糖盐法提取最佳工艺条件为:NaCl浓度2.9％,料液比1∶30 (g/mL),提取时间1.8 h,提取温度80℃,在此条件下多糖得率为1.08％.红外光谱分析表明该多糖结构含有α-型糖苷键连接的吡喃型葡聚糖.抗氧化活性显示该多糖具有一定的还原能力,对羟基自由基(·OH)、1,1-二苯基-2-三硝基苯肼自由基(DPPH·)和2,2'-联氨-双(3-乙基苯并噻唑啉-6-磺酸)二胺盐自由基(ABTS+·)均具有一定的清除效果,清除率与多糖浓度呈正相关.尤其是对ABTS自由基清除效果最佳,当多糖浓度为1.0 mg/mL时清除率可达到80％.吸湿保湿结果显示,该多糖的吸湿保湿性能优于常规的保湿剂壳聚糖和聚乙二醇6000.上述结果表明,盐提得到的虎斑乌贼内脏多糖具有天然抗氧化活性和吸湿保温性能,在功能性食品、药品和化妆品领域具有一定的应用潜力.%The optimal extraction of polysaccharides from Sepia pharaonis viscera was studied using Box-Behnken design,and the antioxidant activities,the moisture-absorption and moisture-retention properties of the polysaccharides were also investigated.The optimal extraction conditions were as follows:NaCl concentration was 2.9％,ratio of water to raw material was 30 mL/g,extraction time was 1.8 h and extraction temperature was 80 ℃.Under the optimal conditions,the yield of polysaccharides was 1.08％.The results of FTIR spectra indicated the polysaccharides was pyranoid glucan with α-glycosidic bond.The polysaccharides was capable of scavenging · OH,DPPH · and ABTS free radicals in a concentration dependent manner in vitro,especially in scavenging ABTS radical,the scavenging ratio reached almost 80％ when the concentration was 1.0 mg/mL.Furthermore,the polysaccharides had certain moisture-absorption and moisture-retention abilities as compared to PEG-6000,glycerol and chitosan.These results indicated that the polysaccharides could be of potential utility in food,pharmaceutical and cosmetic industries as a type of effective natural antioxidant and moisturize.