以无花果叶为原料,对乙醇沉淀、单宁沉淀和盐析沉淀3种方法提取的无花果蛋白酶进行了比较.结果表明,乙醇沉淀法操作简单,提取的无花果蛋白酶易分离纯化,比酶活最高,蛋白酶的回收率达47.99%,总纯化系数为2.05.无花果蛋白酶反应的最适温度为65℃,最适pH值为7.0,当pH值为6.5和8.5时酶活性稳定,Km值约为0.6560g/L.%Three methods of extracting ficin from titus leaves including ethyl alcohol, tannin and sulfate precipitation were compared to explore the most suitable conditions for industrial production of f'mcin. The result indicated that the recovery ratio of enzyme obtained by alcohol was as high as 47.99％; and the purification fold was 2.05; thus ethyl alcohol precipitation was the best extraction method. The optimal pH for enzyme activity of fincin was 7.0; and the optimal temperature was 65℃.The enzyme exhibited most stable activity at pH 6.5 and 8.5, and its Km value was 0.656 0 g/L.
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