白英不同生长期不同药用部位绿原酸与咖啡酸及芦丁含量测定

摘要

目的 探讨白英不同生长期不同药用部位绿原酸、咖啡酸及芦丁含量,为确定其最合适的采收季节提供理论依据.方法 于5,6,7,8,9,10,11月采集白英根、茎、叶、果样品,采用高效液相色谱法测定.色谱条件:Angilent Zorbax ODS C18色谱柱(250 mm×4.6 mm,5μm),柱前加保护柱；流动相为乙腈-1％醋酸溶液,梯度洗脱；流速:0.6 mL·min-1;柱温:25℃；检测波长327 nm.结果 白英根、茎、叶、果实等不同部位绿原酸含量均较高,含量符合以下规律:果实＜下部茎＜根＜上部茎＜叶；不同生长周期根、茎中绿原酸:根部位8月达到最高值(2.583 mg·g-1),下部茎9月达到最大(2.762mg·g-1),上部茎中8月达到最大(1.788 mg·g-1)；叶中绿原酸、咖啡酸、芦丁含量均较高,绿原酸在11月叶中达到峰值(8.169 mg·g-1),咖啡酸在8月叶中达到峰值(0.397 mg·g-1),芦丁在11月叶中达到峰值(3.777 mg·g-1).结论 白英不同药用部位不同生长期绿原酸、咖啡酸及芦丁含量随着生长发育进程而发生变化,综合考虑,以8～9月枝叶茂盛时采收,有效成分含量最高.%Objective To study contents of chlorogenic acid, caffeic acid and rutin in different medicinal parts and growth stage of Solarium lyratum Thunb. And to provide a theoretical basis for confirming an optimal recovery season. Methods The root, stem, leaf and fruit samples were collected from May to November. High performance liquid chromatography (HPLC) method was used. The Angilent Zorbax ODS C18 columm (4. 6 mm ×250 mm, 5μm) was used. The detection wavelength was 327 nm. The mobile phase consisted of acetonitrile-1 % acetum, and the flow rate was 0. 6 mL · min-1. The column temperature was set at 25 ℃. Results Chlorogenic acid in different medicinal parts of Solarium lyratum Thunb. Was comparatively abundant, the distribution of chlorogenic acid content met the following rules: fruit