气相色谱-质谱联用测定复方口腔溃疡贴膜剂中甲硝唑含量

摘要

Objective To develop a method for the determination of metronidazole in complex oral ulcer paster by gas chromatography-mass spectrometry in selective ion monitoring. Methods The metronidazole was analyzed quantitatively on the DB-5 quartz capillary chromatographic column by selective ion monitoring. The sample was extracted in ethanol. Results The linear range of metronidazole was 5-400 μg · mL-1(r = 0. 999 4) , the average recovery was 96.0% (RSD = 2.08% , n = 6) and the detection limit was 1 ng · mL-1 . Conclusion The method is convenient, of good sensibility and accuracy; it can determine metronidazole in complex oral ulcer paster.%目的 建立测定复方口腔溃疡贴膜剂中甲硝唑含量的气相色谱-质谱联用法.方法 选择离子模式检测,以DB-5(30 m×0.32 mm,0.25 μm)石英毛细管为色谱柱,高纯氦气为载气,程序升温.结果 复方口腔溃疡贴膜剂中甲硝唑线性范围为5～400 μg·mL-1(r=0.999 4),平均回收率96.0％(RSD=2.08％,n=6),检测限为1 ng· mL-1.结论 该方法简便,快速,灵敏度高,结果准确可靠.