Objective To study the effect of different conditions on forced swimming test in BALB/C mice, and to provide guidance to enhance the sensitivity, validity and reliability of mice model of depression. Methods The immobility time of BALB/C mice under different conditions (circadian rhythm, water temperature) were investigated. Results The immobility time of BALB/C mice during the daytime is significantly longer than that at night. The differences of the immobility time between male and female mice showed no statistically significant difference, but positive results in male mice was significantly more than that in female mice. The immobility time under water temperature of 22℃ is significantly longer than that under water temperature of 12℃ or 32℃. Conclusion To increase the sensitivity and reliability, the forced swimming test should be taken during the daytime in male BALB/C mice. BalB/C mice are more prone to depression under water temperature of 22℃.%目的 研究不同条件对BALB/C小鼠强迫游泳实验的影响,对该品系小鼠抑郁模型的敏感性、有效性、可靠性进行探索.方法 将不同性别、体重相近的BALB/C小鼠放置在相同的饲养、实验环境下,通过设置不同研究参数(昼夜和水温),对强迫游泳行为进行观察分析.结果 ①雌雄小鼠间的不动时间差异无统计学意义,然而雄性小鼠的阳性结果数多于雌性；②在昼夜节律变化的强迫游泳试验中BALB/C小鼠白天的不动时间显著长于夜间的不动时间；③BALB/C小鼠在22℃水温的不动时间显著长于在12℃、32℃水温的不动时间.结论 为增加强迫游泳实验的敏感性、可靠性,应选择雄性BALB/C小鼠在白天进行试验,在22℃水温下更容易产生抑郁样行为.
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