Strains of Staphylococcus aureus were isolated and identified from raw milk by traditional taxonomic identification and polymerase chain reaction (PCR), and their toxin producing ability was detected by mini-VIDAS to study the S. aureus contamination in raw milk. The results showed that S. aureus was the main contamination bacteria, 9 strains of them were positive which detected by miniVIDAS, and the detection rate of toxigenic strains was 12.86% in the 70 strains of S. aureus isolated. The PCR designed targeting the primer of thermonuclease(nuc) gene of S. aureus had coincidence rate of 100% with traditional taxonomic identification, and it had higher specificity and sensitivity.%为了蟹原料奶中产毒型金黄色葡萄球菌的污染情况,采用传统分类学鉴定方法与PCR鉴定方法结令,对原料奶中金黄色葡萄球菌进行分离鉴定,并运用全自动荧光免疫分析仪检测了分离.菌株的产毒情况.结果表明,分离鉴定的70株金黄色葡萄球菌中,mini-VIDAS仪分析为阳性的有9株,产毒型金黄色葡萄球菌的检出率为12.86%,金黄色葡萄球菌为原料奶的主要污染菌之一.针对金黄色葡萄球菌耐热核酸酶基因设计的引物进行的PCR鉴定方法,与传统分类学方法的符合率为100%,并且具有较高的特异性及敏感性.
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