香樟果皮花色苷提取工艺优化及抗氧化研究

摘要

The extraction technology of camphor pericarp anthocyanins was optimized preferably.The alcohol was used as the extracting solvent.Extracting time and temperature,solvent concentrations,proportion of material and solution and pH were discussed.The model of three factors and three levels was established through box-Behnken.The design of experiment was made by Design-Expert 8.0 software and optimization of various factors and their interaction effects were analyzed by response surface method.The ability of antioxidant was determined with colorimetric method using salicylic acid and DPPH method,and comparison of time to influence the stability of the anthocyanins.The regression equation of the best extraction of anthocyanins was Y = 58.64 + 2.27A + 12.78B +10.18C -14.01A 2 -11.00B 2 -7.56C 2 ,R 2 = 0.9796,and the fitting degree of model was good.Within the range of the test,the model reflected the extraction results.The results showed that the best extraction parameters were pH 1.0,material-solvent ratio 1 g∶15 mL,ethanol content 78.59%,extraction temperature 77.14 ℃,extraction time 42.48 min.On this extraction condition,the content of the camphor pericarp anthocyanins was 67.99 mg·100 g-1 .In a certain concentration,the ability of scavenging hydroxyl free radical and the total antioxidant capability were allpositive linear correlation with the concentration of anthocyanins.The regression equation respectively were y =0.3388x +13.485(R 2 =0.9856),y =0.0275x +0.0221,(R 2 =0.9966).To determine the optimum technological con-ditions by using the response surface method were reasonable,it would provide certain guiding significance for the de-velopment and ntilization of natural pigment.%为了优化香樟果皮花色苷的最佳提取工艺条件,用乙醇作为提取溶剂,选取提取时间、提取温度、提取剂浓度、料液比、pH 五因素,采用 Box-Behnken 建立三因素三水平模型,用响应面法优化各因素及其相互作用的最佳组合,用 Design-Expert 8．0设计回归正交实验；同时用水杨酸比色法和 DPPH 法,测定了香樟果皮花色苷的抗氧化能力,并比较了不同放置时间对香樟果花色苷稳定性的影响.结果表明：香樟果花色苷最佳提取工艺的回归方程为Y ＝58．64＋2．27A ＋12．78B ＋10．18C －14．01A 2－11．00B 2－7．56C 2,R 2＝0．9796,模型拟合程度良好,在该试验范围内,模型能够准确反映花色苷的提取结果；最佳工艺参数分别为 pH1．0、料液比1∶15、乙醇浓度78．59％、提取温度77．14℃、提取时间42．48 min,在此条件下香樟果花色苷的得率为67．99 mg·100 g-1；在一定浓度范围内,香樟果皮花色苷清除羟自由基率和总抗氧化能力均与浓度成正线性相关,回归方程分别为 y ＝0．3388x ＋13．485(R 2＝0．9856),y ＝0．0275x ＋0．0221,(R 2＝0．9966).利用响应面法确定的最佳工艺条件合理,可用于香樟果皮花色苷的提取,同时香樟果花色苷具有良好的抗氧化活性,为天然色素的开发利用提供一定指导意义.