Isolation and determination of major anthocyanin pigments in the pericarp of P. communis L. cv. Red Du Comices and their association with antioxidant activity

摘要

The anthocyanins in the fruit skin of?Pyrus communis?cv. ‘Red Du Comice’ were isolated, identified and quantified using high-performance liquid chromatography/diode array detection (HPLC/DAD) and HPLC/electrospray ionization/mass spectrometry (HPLC/ESI/MS). The individual anthocyanins were identified by comparing their mass spectral data and retention times with those of standards and published data. Cyanidin-3-galactoside was the major compound, taking up to 93% of the total anthocyanin content. The content of Cyanidin-3-galactoside reached 23.7 ± 3.2 mg 100 g-1?fresh weight, followed by the eighth peak (2.14%) and Cyanidin-3-glucoside (1.23%). Pelargonidin-3-rutinoside (pg-3-rutinoside) was identified for the first time in?P. communis?L. 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays showed that the extract from the fruit peel of ‘Red Du Comice’ contained anthocyanins and possessed high antioxidant capacity.