以采自福建省山区的正红菇 Russula griseocarnosa 为研究材料，进行 ITS （ internal transcribed spacer）、RPB2（the second largest subunit of the nuclear RNA polymerase enzyme II ）保守区段克隆测序和序列同源性比对。将从 GenBank 中获得的部分红菇属物种 ITS 和 RPB2序列与正红菇序列通过最大简约法进行系统发育分析。基于 ITS 序列构建的系统发育树表明，福建正红菇与云南大红菌 Russula griseocarnosa X ．H ．Wang ，Zhu L ． Yang ＆ Knudsen ，sp ．nov ．间序列差异较小，亲缘关系较近，以99％的支持率聚为一簇。福建正红菇与欧洲红菇Russula vinosa 及玫瑰红菇 Russula rosea 间序列差异较大，亲缘关系较远，在系统发育树上聚为不同分枝。基于RPB2序列构建的系统发育树也表明福建正红菇与云南大红菌聚为一簇（支持率94％），而与玫瑰红菇聚为不同分支。%Fruiting bodies of Russula griseocarnosa were collected in Fujian to study the mushroom′s phylogenetic diversity .Two gene regions of ITS ( internal transcribed spacer) and RPB2(the second largest subunit of the nuclear RNA polymerase enzyme II ) were cloned .The phylogenetic diversity was examined according to the maximum parsimony trees with sequences of several other Russula species .It showed that ,based on the ITS sequences ,R . griseocarnosa found in the province clustered with R .griseocarnosa X .H .Wang ,Zhu L .Yang & Knudsen ,sp .nov . found in Yunnan ,with a bootstrap value of 99% .The differences among R .griseocarnosa ,R .vinosa and R .rosea were apparent ,with a bootstrap value below 50% .The maximum parsimony tree based on the RPB2 sequences also showed that R .griseocarnosa clustered with R .griseocarnosa X .H .Wang , Zhu L .Yang & Knudsen , with a bootstrap value of 94% .The distinction was also apparent ,with a bootstrap value less than 50% .
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